Which of the following is true about the Edman degradation system of sequencing polypeptides?
Which of the following is true about the Edman degradation system of sequencing polypeptides?
A. The Edman degradation system will work on any size polypeptide.
B. In the Edman degradation system the amino-terminal residue is labeled and the polypeptide is hydrolyzed to its constituent amino acids.
C. In the Edman degradation system the amino-terminal residue is labeled, cleaved and identified in each successive cycle.
D. The Edman degradation system is carried out on a machine called an edmanator.
参考答案:C
The polypeptide is treated with a reagent that labels the amino-terminal residue, then that residue is removed and identified. In the next cycle, the identity of the new amino-terminal residue is similarly determined, and the procedure is repeated until the entire sequence is determined.